|
Optimization of alkaline
protease production from Pseudomonas fluorescens
isolated from meat waste contaminated soil
K. Kalaiarasi* and P. U.
Sunitha
Department of Textiles and Clothing, Avinashilingam
University for Women, Coimbatore 641 043, Tamil Nadu, India.
*Corresponding author. E-mail:
kalaiarasik@rediffmail.com.
Accepted
17 March, 2009 |
|
A protease
producing bacteria was isolated from meat waste contaminated
soil and identified as Pseudomonas fluorescens.
Optimization of the fermentation medium for maximum protease
production was carried out. The culture conditions like
inoculum concentration, incubation time, pH, temperature,
carbon sources, nitrogen sources and metal ions were
optimized. The optimum conditions found for protease
production was 37ºC at pH 9, with 2% inoculum in the medium
for 24 h. Wheat bran and peptone stimulates the production
of protease. Magnesium sulphate has less inhibitory effect
among the metal ions tested.
Key
words:
Alkaline protease, casein agar, meat waste contaminated
soil, Pseudomonas fluorescens. |
