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Rooting success using IBA
auxin on endangered Leucadendron laxum (PROTEACEAE)
in different rooting mediums
C. P. Laubscher
and P. A. Ndakidemi*
Faculty
of Applied Sciences, Cape Peninsula University of
Technology, P. O. Box 652, Cape Town 8000, South Africa.
*Corresponding author. E-mail: ndakidemip@cput.ac.za. Tel:
+2721-460-3196. Fax: +2721-460-3193.
Abbreviations: IBA, Indole-3-butyric acid; ppm,
parts per million; and SAFEC, South African Flower
Export Council.
Accepted
28 July, 2008 |
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Leucadendron laxum
(Marsh rose Leucadendron) was tested for its rooting
ability as an endangered plant species for reintroduction
into the natural habitat, using IBA liquid hormone
preparations and four growth media. The treatments included
a control, 500, 1000, 2000 and 4000 ppm concentrations. Four
growth media, namely:
bark and polystyrene; peat moss and polystyrene; bark, river
sand and polystyrene;
and perlite and river sand
were
tested in an environmentally controlled greenhouse with
bottom heat. The experiments used a randomised block design
with 10 cuttings per treatment and 4 replicated four times.
The results showed that the bark, sand and polystyrene
medium had the
highest (45%) significant survival rate when supplied with
IBA at 500 ppm. The application of 1000 ppm of IBA in
bark,
sand and polystyrene medium significantly increased the
number of roots per cutting (5) and produced the longest
roots (4 cm). The bark and polystyrene medium supplied with
zero IBA had the largest number of roots per cutting (6.5).
The comparison of the four growth media showed that bark and
polystyrene was the best medium that produced the highest
and most significant callusing formation (30%), % of
cuttings that rooted (28%), cuttings with a higher survival
rate (57%), and cuttings with a higher number of roots (3.5)
and the longest roots (7.35 mm). With regard to various IBA
treatments tested across all treatments, the IBA supplied at
1000 ppm produced greater root numbers when compared with
all other treatments.
Key words:
Hormone treatment, red data species, rooting percentage,
vegetative propagation. |
