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Screening of endophytic
fungi that promote the growth of Euphorbia pekinensis
Chuan-chao Dai1*,
Bo-yang Yu2 and Xia Li3
1Jiangsu
Key Laboratory for Diversity and Biotechnology, College of
Life Science, Nanjing Normal University, Nanjing , Jiangsu
Province, 210046, China.
2Department
of Complex Recipe of CMM, China Pharmaceutical University,
Nanjing, Jiangsu Province, 210038, China.
3Institute
of Foods Crop, Jiangsu Academy of Agricultural Science,
Nanjing, Jiangsu Province, 210014, China.
*Corresponding author. E-mail:
daichuanchao@njnu.edu.cn.
Accepted
25 August, 2008 |
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This
study explored a strategy to use endophytic fungi for
promoting the growth of the medicinal plant, Euphorbia
pekinensis. The growth of
E. pekinensis was examined in pot culture
following inoculation of E. pekinensis
with
endophytic fungal strains (Fusarium spp.) from
E. pekinensis (E4 and
E5) and those not from E.
pekinensis (B3, B6 and
S12). The results showed that plants treated
with E4 or E5 exhibited significant increase in growth and
all tested growth parameters, as compared to the control
(CK). The biomass of E4- and E5-treated plants was 2.08 and
1.50 fold higher than that of CK. Analysis of superoxide
dismutase (SOD) activity, the indicator of unsaturated fatty
acids (IUFA), showed a similar trend as the growth biomass,
indicating that the treatments of E4 and E5 benefited the
growth of the host plantlets. Indole acetic acid (IAA) and
gibberellin (GA) were found in the fermented broth of E4 and
E5. E. pekinensis cell suspensions demonstrated
similar responses to treatment with mycelia extract or
filtrate of E5 culture medium. Replacement of MS culture
medium components with E5 extracts in host cell suspensions
suggested that E5 extract could replace auxin to enhance the
plant growth. Therefore, strain E5 could be used as a
growth-promoting strain for E. pekinensis. The
method used in this study could be applicable to similar
studies on the relationship between endophytic fungi and
their host plants. This method provides a technique to
accelerate the growth of E. pekinensis plantlets and
suspension cells.
Key
words:
Euphorbia pekinensis, endophytic fungi, Fusarium
spp., growth promotion, phytohormone. |
