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Full Length Research Paper
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Evaluation of in vitro
Vibrio static activity of Shewanella algae
isolated from healthy Penaeus monodon
Shahram Shakibazadeh, Che
Roos Saad*, Annie Christianus, Mohd Salleh Kamarudin,
Kamaruzaman Sijam, Mariana Nor Shamsudin and Vasantha Kumari
Neela
Department of Aquaculture, Faculty of Agriculture,
University Putra Malaysia, 43400 Serdang, Selangor,
Malaysia.
*Corresponding author.
E-mail:
cheroos@agri.upm.edu.my,
cheroossaad@yahoo.com,
Tel: 006-03-8946-4119 & 006-016-717-7436, Fax:
006-03-8946-4146
Accepted 7 October, 2008 |
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Abstract |
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To conquer disease problem in shrimp industries, probiotic
biocontrol is a well known remedy now. The antagonistic
ability of separated isolates from different parts of
juvenile Penaeus monodon were screened against shrimp
Vibrio pathogens; Vibrio parahaemolyticus and
Vibrio alginolyticus. The most antagonistic effect
was observed for an isolate that was primarily identified as
Shewanella algae using conventional methods followed
by Biolog microlog software. Since production of
antagonistic agents rely on cultural conditions,
antagonistic ability of candidate probioic against the
mentioned Vibrios was assessed using Response Surface
Methodology, with central composite design in which four
independents variables were assumed: temperature (10 -
50°C), pH (6 -10), NaCl concentration (0 - 50%) and time (12
– 60 h). The coefficients of multiple determinations (R2),
for the responses of antagonistic effect of S. algae
against V. parahaemolyticus and V. alginolyticus
values were 0.807 and 0.805, respectively. Concentration of
the NaCl exhibited least influence on the antibacterial
effect of candidate probiotic while the other independent
variables exhibited different degree of affect. The
candidate probiotic revealed a reasonable antibacterial
response in quite a wide range of temperature and pH in
which the maximum levels were in the same range of optimum
shrimp culture.
Key
words:
Antagonism, Penaeus monodon, probiotic, Shewanella
algae, Vibrio parahaemolyticus, Vibrio
alginolyticus. |
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