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Biosynthesis of
cellulolytic enzymes by Tricothecium roseum with
citric acid mediated induction
Prashanth Shanmugam*,
Madhumathi Mani and Mathivanan Narayanasamy
Center for Advanced Studies in Botany, University of
Madras, Chennai, 600025, India.
*Corresponding author. E-mail:
prashmicrobiol@yahoo.co.in.
Accepted
15 August, 2008 |
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Tricothecium roseum, an unexplored fungus for the production of cellulolytic
enzymes, was used in this study for carboxymethylcellulase (CMcase)
and
b-glucosidase production. The culture filtrate of this
organism exhibited highest total cellulolytic activity and
extracellular protein content on 9th day during
the course of its growth on potato dextrose yeast extract (PDYE)
broth medium supplemented with 1.0% (w/v)
carboxymethylcellulose. Among various soluble carbon and
complex nitrogen sources tested in this study,
caboxymethylcellulose and peptone supported maximum
production of both cellulolytic enzymes. Under all suitable
growth conditions, the enzyme biosynthesis was remarkably
increased when the inducer citric acid was added to the PDYE
media containing carboxymethyl-cellulose. Maximum CMcase
production (1.59 U/ml) and
b-glucosidase
production (1.82 U/ml) were achieved in citric acid amended
PDYE media at 37°C. Both the cellulolytic enzyme production was highest
at initial pH 6.5 and after 7 days incubation. The study
clearly demonstrated that citric acid is a good inducer for
extracellular cellulolytic enzyme production by the fungus.
Key words: Tricothecium roseum, cellulolytic enzymes, CMcase,
b-glucosidase. |
