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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 7 No. 21

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  Search Pubmed for articles by:

  Keshtkar AR
  Dalfardi S

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African Journal of Biotechnology Vol. 7 (21), pp. 3874–3877, 5 November 2008

ISSN 1684-5315  © 2008 Academic Journals  

 

 

Full Length Research Paper

 

Methods to break seed dormancy of Astragalus cyclophyllon

 

A.R. Keshtkar, H. R. Keshtkar*, S. M. Razavi and S. Dalfardi

 

Faculty of Natural Resources, University of Tehran, Karaj, Iran.

 

*Corresponding author. E-mail: hkeshtkar97@yahoo.com.

 

Accepted 20 August, 2008

 
   Abstract
 

The aim of this study was to enhance the germination rate of Astragalus cyclophyllon seeds which have a very low germination rate under normal conditions. The seeds were soaked for 72 h in 100, 200, 300, 400 and 500 ppm gibberellic acid (GA3) solution, H2SO4 concentrations (50 and 98%) at two treatment times (5 and 10 min) and 60, 80 and 100șC hot water at two treatment times (5 and 10 min) before placing in Petri dishes. The fresh seeds (non-stratified) of A. cyclophyllon had 55% germination. Analysis of variance indicated that both GA3 and H2SO4 concentrations had significant effects on seed germination and final germination percentage. The highest germination percentage (81%) was obtained when the seeds were treated with 500 ppm GA3. The results showed that hot water treatments are not useful methods for breaking the seeds dormancy.

 

Key words: Chemical stratification, gibberellins, mechanical scarification.

 

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