Full Length Research Paper

In vitro micro-propagation of endangered ornamental plant-Neotchihatchewia isatidea (Boiss.) Rauschert

Ahmet Gümüşçü^1*, Satı Çöçü², Serkan Uranbey³, Arif İpek⁴, Mikail Çalışkan³ and Neşet Arslan⁴

¹Selçuk University Çumra Educational High College, Turkey.

²Department of Field Crops, Faculty of Agriculture, University of Erciyes Kayseri, Turkey.

³Central Research Institute for Field Crops, Yenimahalle, Ankara, Turkey.

⁴Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110, Dışkapı, Ankara, Turkey.

*Corresponding author. E-mail: agumuscu19@yahoo.com.

Abbreviations: MS, Murashige and Skoog medium; BAP, N⁶-benzylamino-purine; NAA, α-naphthaleneacetic acid; KIN, Kinetin, IBA, indole-3-butyric acid; IAA, indole-3-acetic acid; and TDZ, thidiazuron.

Accepted 2 January, 2008

The ornamental plant, Neotchihatchewia isatidea, is an endangered species of Turkey and threatened by complete extinction in the future. Therefore, in vitro multiplication of this species can be valuable for commercial production and germplasm conservation. Immature embryos of N. isatidea were cultured for initiation on Murashige and Skoog medium (MS) supplemented with N⁶-benzylamino-purine (BAP) and α-naphthaleneacetic acid (NAA). Shoot primordia were visible within 5 - 6 weeks and the shoot primordia later developed into normal shoots 10 - 12 weeks after the culture initiation on calli developed from immature embryos. Shoot tips were also excised from developed plantlets for direct shoot organogenesis and cultured on MS shoot induction medium supplemented with BAP (0.5, 1.0 and 2.0 mg/l), kinetin (KIN) (0.5, 1.0 and 2.0 mg/l) and thidiazuron (TDZ) (0.05, 0.10 and 0.50 mg/l). Direct multiple shoots from shoot tips developed in most media tested. High shoot multiplication (3.73), high rooting (53 %) number of root per shoot (3.66) and survival ratio (46.6 %) were achieved. 

Key words: Neotchihatchewia isatidea, shoot regeneration, immature embryo, shoot tip, rooting.