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14C-Glucose
uptake studies in the red rot toxin treated sugarcane callus
A. B. Vedamurthy1,
M. K. Sateesh2, G. R. Naik3
1Post
Graduate Department of Biotechnology, The Oxford College of
science, J.P Nagar, Bangalore-560078, India.
2Department
of Biotechnology and Microbiology Bangalore University,
Jnana Bharathi, Bangalore 560056, India.
3Department
of Biotechnology Gulbarga University, Gulbarga-585106,
India.
*Corresponding author. Email:
vedamurthy15@yahoo.co.in
Abbreviations:
PPO,
5-Diphenyl Oxazole; POPOP, 1,4-Bis(5-Phenyl Oxazolyl)
Benzene.
Accepted 8
January, 2008 |
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Fungal toxins cause serious damage to the cellular functions
of host tissue. In the present report the toxin extracted
from Colletotrichum falcatum Went was partially
purified and treatments were given to the callus of
susceptible sugarcane callus variety CoC 671. The influence
on 14C-glucose uptake and its further utilization
was investigated. The toxin treatment reduced the total
uptake of glucose and also inhibited its conversion into
insoluble products of cellular metabolism. Accumulation of
organic acids and lowered synthesis of total sugars was
mainly noticed. The susceptibility of sugarcane var. CoC 671
to red toxin seems to be due to poor efficiency and
imbalance in utilization of glucose under biotic influence.
Key
words:
Colletotrichum falcatum,
fungal toxin, autoradiogram, callus. |
