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  Afr. J. Biotechnol.

  Vol. 7 No. 5

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  Search Pubmed for articles by:

  Kumar A
  Jain VK

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African Journal of Biotechnology Vol. 7 (5), pp. 644–650, 4 March 2008

ISSN 1684-5315  © 2008 Academic Journals  

 

 

Full Length Research Paper

 

Solid state fermentation studies of citric acid production

 

Ashish Kumar1* and V. K. Jain2

 

1Central Research & Development Laboratory, Hindustan Zinc Limited, P.O. Zinc Smelter, Debari, Pin 313024, Dist. Udaipur, Rajasthan, India.

2Department of Biochemical Engineering and Food Technology, H. B. Technological Institute, Kanpur-208002, U.P., India.

 

*Corresponding author. E-mail: ashish.be@gmail.com. Tel: +91-294-2655618, 2656612, extn 240. Fax No. +91-294-2655057.

 

Accepted 27 November, 2007

 
   Abstract
 

Treated sugarcane bagasse supplemented with sucrose medium was found 1.7 fold (citric acid based on sugar consumption) better substrate than untreated bagasse carrier. The performance of packed bed reactor at aeration rate of 0.75 l/min and apparent packing density of 35.0 g/l was superior with citric acid yield of 55.90% (w/w), overall productivity of 0.087 g/100 g DS.h and specific growth rate of 0.055 h-1. However, in flask fermentation citric acid yield of 41.56% (w/w) with overall productivity of 0.064 g/100 gDS.h and specific growth rate of 0.043 h-1 was observed. The system confirmed that citric acid production was Type-II fermentation. Citric acid recovery of 90.39% (w/w) was achieved from fermented broth.

 

Key words: Aspergillus niger, kinetics, packed bed reactor, sugarcane bagasse.

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