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Solid state fermentation
studies of citric acid production
Ashish Kumar1*
and V. K. Jain2
1Central
Research & Development Laboratory, Hindustan Zinc Limited,
P.O. Zinc Smelter, Debari, Pin 313024, Dist. Udaipur,
Rajasthan, India.
2Department
of Biochemical Engineering and Food Technology, H. B.
Technological Institute, Kanpur-208002, U.P., India.
*Corresponding author.
E-mail:
ashish.be@gmail.com.
Tel: +91-294-2655618, 2656612, extn 240. Fax No.
+91-294-2655057.
Accepted
27 November, 2007 |
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Treated sugarcane bagasse supplemented with sucrose medium
was found 1.7 fold (citric acid based on sugar consumption)
better substrate than untreated bagasse carrier. The
performance of packed bed reactor at aeration rate of 0.75
l/min and apparent packing density of 35.0 g/l was superior
with citric acid yield of 55.90% (w/w), overall productivity
of 0.087 g/100 g DS.h and specific growth rate of 0.055 h-1.
However, in flask fermentation citric acid yield of 41.56%
(w/w) with overall productivity of 0.064 g/100 gDS.h and
specific growth rate of 0.043 h-1 was observed.
The system confirmed that citric acid production was Type-II
fermentation. Citric acid recovery of 90.39% (w/w) was
achieved from fermented broth.
Key
words:
Aspergillus niger, kinetics, packed bed reactor,
sugarcane bagasse. |