|
Development
of a high-throughput microsatellite typing approach for
forensic and population genetic analysis of wild and
domestic African Bovini.
B.J. Greyling1,2*,
P. Kryger1,5, S. du Plessis2, W.F. van
Hooft1,6, P. van Helden3, W.M. Getz1,4
and A.D.S. Bastos1
1Mammal
Research Institute, Department of Zoology and Entomology,
University of Pretoria, Pretoria 0002, South Africa.
2Agricultural
Research Council, Irene, South Africa.
3Division
of Molecular Biology and Human Genetics,
University of Stellenbosch, South Africa.
4Department
of Environmental Science Policy and Management, University
of California at Berkeley, USA.
5Department
of Integrated Pest Management, Research Centre Flakkebjerg,
Denmark
6Resource
Ecology Group, Wageningen University, Droevendaalsesteeg 3a,
building nr. 100, 6708 PB, Netherlands
*Corresponding author. E-mail:
Ben@arc.agric.za. Tel:
+2712 672-9393. Fax: +2712672-9214.
Accepted 23 January, 2008 |
|
Conservation management and forensic traceability of African
buffalo and cattle rely on the timely provision of unbiased
and accurate genetic information. An approach in which 17
cattle microsatellite markers are co-electrophoresed,
following amplification in three core multiplex reactions
was established for this purpose. Mean allelic richness per
locus was 8.24 and 6.47, for buffalo and Bonsmara cattle,
respectively, whilst
an
unbiased match probability of
6.5x10-17 and 1.03 x 10-16 was obtained for each.
These results confirm the usefulness of this rapid,
cost-effective typing approach for forensic, paternity and
fine-scale genetic analyses of wild and domestic African
Bovini tribe members.
Key
words:
African buffalo,
microsatellite, forensic, traceability, typing, Bovini,
Africa. |
