Full Length Research Paper

Autofluorescence of routinely hematoxylin and eosin-stained sections without exogenous markers

Deeb S.*, Nesr K. H., Mahdy E., Badawey M. and Badei M.

Department of Pathology, Faculty of Veterinary Medicine, University of Beni Suef, Egypt.

*Corresponding author. E-mail: salahdeeb40@yahoo.com.

Accepted 16 January, 2008

Hematoxylin and eosin stained paraffin section was examined by fluorescence microscopy to study the pattern and distribution of fluorescence.  Autofluorescence was sensitive and specific for detection of elastic and collagen fibers. It was concluded that analytical morphological techniques based on autofluorescence can obtain information about morphological and pathological state of tissue and cells.

Key words: Histology, pathology, fluorescence microscopy.