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Isolation of flavonoids
from mulberry (Morus alba L.) leaves with macroporous
resins
Jun Wang, Fu An Wu*, Hui
Zhao, Li Liu and Qiu Sheng Wu
College of
Biotechnology and Environmental Engineering, Jiangsu
University of Science and Technology, Sericultural Research
Institute, Chinese Academy of Agricultural Sciences
Zhenjiang 212018, P.R. China.
*Corresponding
author. E-mail:
fuword@163.com. Tel: 86-511- 8561-6571.
Accepted
20 May, 2008 |
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Flavonoids are major active constituents in
mulberry (Morus alba L.) leaves and possess various
pharmacological activities. In the present study, the
extraction technology and preparative separation of total
flavonoids (TFs) in mulberry leaves extracts by macroporous
resins were studied systematically, and the column packed
with selected resin was used to perform dynamic adsorption
and desorption tests to optimize the separation process. The
research results indicated that the maximal yield of TFs was
achieved when mulberry leaves were extracted under the
optimized conditions of a pH of 8, a solid-liquid ratio of
1:40, a temperature of 90oC and a time of 4 h.
H103 resin is the most appropriate for the separation of TFs
from other components in mulberry leaves extracts, which
adsorption behavior can be described with Langmuir and
Freundlich isotherms and two-step adsorption kinetics model.
The optimum parameters for TFs separation by H103 were
achieved, after one run treatment. The recovery and purity
of TFs in the final product were 90.57 and 76.33%,
respectively. In conclusion, the preparative separation of
TFs in mulberry leaves can be easily and effectively done by
H103 resin.
Key words:
Optimization, isolation, flavonoids, Morus alba (L.),
mulberry leaves, macroporous resin. |
