Short Communication

Screening and characterization a RAPD marker of tobacco brown-spot resistant gene

H. Y. Zhang¹, Y. M. Yang¹, F. S. Li², C. S. He³ and X. Z. Liu¹*

¹Biotechnology Laboratory, Southwest Forestry College, Kunming, 650224 Yunnan Province, People’s Republic of China.

²College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201Yunnan Province, People’s Republic of China.

³The South Center of Tobacco Breeding Research of China, Yuxi, 653100 Yunnan, People’s Republic of China.

*Corresponding author. E-mail: hanyaoz@163.com. Tel: +86-0871-3863022. Fax: +86-0871-3862178.

Accepted 2 July, 2008

Bulk Segregant Analysis (BSA) and Randomly Amplified Polymorphic DNA (RAPD) methods were used to analyze F₂ individuals of 82-3041 × Yunyan 84 to screen and characterize the molecular marker linked to brown-spot resistant gene. A total of 800 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Primer S361, producing one RAPD marker S361₆₅₀, was tightly linked to the brown-spot resistant gene. Linkage analysis was carried out using marker S361₆₅₀ on 1042 individuals of F₂ progenies from the crossing between 82-3041 × Yunyan 84. The results demonstrated that the genetic distances between S361₆₅₀ and brown-spot resistant gene was 2.98 cM.

Key words: Brown-spot, resistance gene, bulk segregant analysis, molecular marker, and linkage analysis.