|
Plant regeneration as affected by plant growth regulators
(PGR) in mangosteen (Garcinia mangostana L.)
Mohammad Hossein Torabi Sirchi*, M. A. Kadir, M. A. Aziz, A.
A. Rashid, Arash Rafat and M. B. Javadi
Department of Agriculture Technology, Faculty of
Agriculture, Universiti Putra Malaysia, 43400 Serdang,
Selangor Darul Ehsan, Malaysia, C/p: +60176066149.
*Corresponding author. E-mail:
hossein955@yahoo.com,
mbjavadi2002@yahoo.com. Tel: 0389464148. Fax:
0389464115.
Accepted 20 June, 2008 |
|
A protocol was developed for in vitro plantlet
regeneration of mangosteen (Garcinia Mangostana L.)
from shoot tip, stem and seed explants. Shoot tip explants
produced the highest mean number of shoots per explant on
media supplemented with 1.0 mg/L 6-benzylaminopurin (BAP)
and 0.05 mg/L kinetin (KIN) (73.3). On medium containing 0.1
mg/L BAP and 0.05 mg/L KIN, the highest mean shoot height
reached 1.2 cm. The highest percentage of callus formed
(90%) was obtained in treatment containing 0.20 mg/L KIN.
The effect of BAP in combination with
α-naphthalene acetic acid
(NAA) was also studied. On half seed explants, the combination of 4 mg/L (w/v) of BAP with 0.2 mg/L (w/v)
of (NAA) produced the highest number of shoots per explant
(75). In medium containing 2 mg/L (w/v) of BAP and 0.2 mg/L
(w/v) of NAA, the mean shoot height reached 1.79 cm also The
highest percentage of callus formation (90%) was obtained on
treatment containing 0.5 mg/L (w/v) of NAA. For rooting,
pretreatment in different indol-3-butyric acid (IBA) and NAA
concentrations and comparison of MS salt strengths were
evaluated. Identical pattern occurred on medium containing
one-quarter strength MS salt and the highest mean number of
roots per explant (1.17) was produced in treatment with 2
mg/L IBA. The highest
percentage of root formation was obtained on one-quarter
strength MS salt medium containing 0.1 mg/L NAA (90.4%).
Rooted plantlets were acclimatized on medium composed of
soil + sand + organic matters + vermiculite according to the
ratio (2: 2: 1: 1).
Key words:
Garcinia Mangostana L., 6-benzylaminopurine,
α-naphthalene acetic acid, Indole-3-butyric acid, kinetin. |
