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Polymorphism of
calpastatin gene in Arabic sheep using PCR- RFLP
M. Mohammadi, M. T. Beigi Nasiri, K h. Alami-Saeid, J.
Fayazi, M. Mamoee and A. S. Sadr*
Ramin
Agricultural and Natural Resources University, Mollasani,
Ahwaz, Iran.
*Corresponding author. E-mail:
Mahsa_mohammadi16@yahoo.com.
Accepted
16 June, 2008 |
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Calpastatin has been known as candidate gene in muscle growth
efficiency and meat quality. This gene has been located to
chromosome 5 of sheep. In order to evaluate the calpastatin
gene polymorphism, random blood sample were collected from
111 Arabic ram sheep from different regions. The DNA
extraction was based on Boom et al. (1989) method. Exon and
entron I from L domain of the ovine calpastatin gene was
amplified to produce a 622 bp fragment. The PCR products
were electrophoresed on 1.2% agarose gel and stained by
etidium bromide. Then, they were digested with restriction
enzyme MspI and then electrophoresed on 2.5% agarose
gel with ethidium bromide and revealed two alleles, allele A
and allele B. Data were analysed using PopGene32 package. In
this population, AA, AB, BB genotype have been identified
with the 70.27, 28.82, 0.9% frequencies. A and B allele’s
frequencies were 0.85, 0.15, respectively. The population
was found to follow Hardy-Weinberg equilibrium.
Key
words:
Calpastatin gene, polymorphism, PCR-RFLP. |
