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Survival of
Newcastle disease virus (NDV) strain V4-UPM
coated on three grains offal and exposed to room temperature
Echeonwu, GON1*,
Iroegbu, CU2, Ngene, A3, Junaid, SA1,
Ndako, J1, Echeonwu, IE4, Okoye, JOA5
1Virology
Department, Federal College of Veterinary and Medical
Laboratory Technology, National Veterinary Research
Institute, Vom, Plateau State, Nigeria.
2Department
of Microbiology, University of Nigeria, Nsukka, Enugu State,
Nigeria.
3Department
of Veterinary Medicine, University of Nigeria, Nsukka, Enugu
State, Nigeria.
4Department
of Veterinary Pathology and Microbiology, University of
Nigeria, Nsukka, Enugu State, Nigeria.
5Department
of Animal Health, Federal College of Animal Health and
Production Technology, National Veterinary Research
Institute, Vom, Plateau State, Nigeria.
*Corresponding author. E-mail:
echeonwugeorgebest@yahoo.com. Tel: +2348033503715,
+2348053288007.
Accepted 18 June, 2008 |
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Newcastle disease virus (NDV) strain V4-UPM
was investigated for its viability when coated on different
grains offal following exposure to room temperature (RT)
(21-27°C) for 8 weeks and using residual infectivity
titration at weekly intervals in chick embryos as a measure
of viability. The grains (maize, sorghum and millet) used
for the study were processed to produce the offal which was
dried in the sun before and at RT under a gentle air current
after coating with virus. The time duration taken for the
infectivity of the virus to drop below the minimum
immunizing dose (MID) (log10 EID50/g ≥
6.0) was compared for virus suspensions containing additive
(2% gelatin) and without additive. Results showed that the
virus coated onto the carrier foods offal without additive
remained stable at ≥ MID value for ≈3 weeks (millet), 3.5
weeks (sorghum) and ≈5 weeks (maize) and with additive for
≈5.2 weeks (sorghum), 5 weeks (millet) and ≈6 weeks (maize)
at RT. Thus, V4-UPM was found in this study to be
stable even without additive for a minimum of 3 weeks on one
of the grains offal, a reasonable time for the food vaccine
to reach remote areas of most villages. It is concluded that
the waste byproducts of any of these foods could be suitable
as carriers for food-based vaccination of rural chickens in
Nigeria.
Key words:
V4-UPM, grains offal, stability, room temperature, storage. |
