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Antibacterial
activity from
Siamese
crocodile (Crocodylus siamensis) serum
Sutthidech Preecharram1,
Sakda Daduang2, Wandee
Bunyatratchata3, Tomohiro Araki4
and Sompong Thammasirirak2*
1Graduate
school, Khon Kaen University, Khon Kaen 40002, Thailand.
2Department
of Biochemistry, Faculty of Science, Khon Kaen University,
Khon Kaen 40002, Thailand.
3Department
of
Microbiology,
Faculty of Science, Khon Kaen University, Khon Kaen 40002,
Thailand.
4Department
of
Bioscience,
School of Agriculture, Kyushu Tokai University, Kumamoto-Aso
869-1404, Japan.
*Corresponding author. E-mail: somkly@kku.ac.th Tel:
+66-43-342911; Fax: +66-43-342911.
Abbreviations:
CFU, Colony forming unit; DDW, sterile
distilled water;
HCCA, α-cyano-4-hydroxy-cinnamic acid;
HPLC,
High Performance Liquid Chromatography; and TFA,
Trifluoro acetic acid.
Accepted 28 July, 2008 |
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Antibacterial agents were purified from Siamese crocodile
serum by anion exchange, gel filtration and reversed phase
HPLC. Six antibacterial agents designed as Hp14,
Hp15, Hp17, Hp31, Hp36 and Hp51 were purified and
proved to carry
activity
against Salmonella typhi, Escherichia coli,
Staphylococcus aureus, Staphylococcus epidermidis,
Klebsiella pneumoniae, Pseudomonas aeruginosa
and Vibrio chorelae. The mass analysis of MALDI-TOF
for
antibacterial agent of Hp14, Hp15 and Hp51 revealed that
they are small molecule with a molecular mass less than 1
kDa. The
scanning electron microscopy
demonstrated that these agents targeted the bacterial
membrane and they act like as antimicrobial peptides. The
antibacterial agent in the serum may represent the first
line of
an immune system
in a freshwater crocodile.
Key words:
Antibacterial agent, crocodile, Crocodylus siamensis,
reptile. |
