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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 7 No. 17

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  Search Pubmed for articles by:

  Pirsaraei ZA
  Mianji GR

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African Journal of Biotechnology Vol. 7 (17), pp. 3149–3154, 3 September 2008

ISSN 1684-5315  © 2008 Academic Journals  

 

 

Full Length Research Paper

 

Effect of testosterone and growth hormone injection before puberty on follicles size, rate of egg production and egg characteristics of the Mazandaran Native breeder hens

 

Z. Ansari Pirsaraei1, A. Zare Shahneh1, M. Zaghari1, M. J. Zamiri2 and G. Rahimi Mianji3

 

1Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Iran.

2Department of Animal Science, Faculty of Agriculture, University of Shiraz, Iran.

3Department of Animal Science, Faculty of Animal Science and Fishery, Sari University of Agricultural and Natural Resources, Iran.

 

*Corresponding author. E-mail: zarbakht_ansari@yahoo.com. Tel: +989111327206.  Fax: +982612246752.

 

Accepted 21 July, 2008

 
   Abstract
 

Egg shell quality and egg internal quality are of major importance to the egg industry worldwide. This experiment was conducted to evaluate the effect of testosterone and growth hormone (hGH) on egg production and characteristics. The aim of this trial is to test this hypothesis that one injection of these two hormones before puberty has any effects on egg production of Mazandaran native breeder hens. Two hundred native pullet used in a completely randomized design with 4 treatments, 5 replicates and 10 hens in each box. Hens were fed on common diet and hormones injected subcutaneously based on body weight (BW). The four treatments were: 1) injection of hGH (100 µg/kg BW), 2) injection of testosterone (500 µg/kg BW), 3) injection of hGH (100 µg/kg BW) + injection of testosterone (500 µg/kg BW) and 4) injection of 100 µl distilled water (control). Injection was before 5% oviposition of flock (flock puberty). Egg production and characteristics were determined. Treatments affected ovary weight, small white, large white and small yellow follicles and some egg parameters. GH and testosterone can alter follicles size in ovary, and it is concluded that GH+testosterone injection is more effective on small and large follicles. 

 

Key words: Testosterone, growth hormone, follicle size, egg production, breeder hen.

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