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Hydrodynamic behaviour and
biochemical characterization of a simple custom expanded bed
column for protein purification
M. H. Shahavi, G. D. Najafpour* and M. Jahanshahi
Faculty of
Chemical Engineering, Babol University of Technology, Iran.
*Corresponding author.
E-mail:
najafpour@nit.ac.ir,
najafpour8@yahoo.com.
Tel:
+(98)111 3234204.
Fax: +(98)111 3234201/ +(98)111 3234204.
Accepted
19 November, 2008 |
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Hydrodynamic behaviour and biochemical characterization of a
simple custom expanded bed column made by Nanobiotechnology
Group (NBG) was compared with a commercial expanded bed
contactor. Hydrodynamic characteristics of the columns (Daxl
and Bo) with various settled bed height (SBH = 5 - 10 cm,
increment of 1 cm) along with variety of column diameters
(1.0, 1.6, 2.0 and 2.5 cm) were investigated. All parameters
were found to be comparable or superior to those reported
for conventional, expanded bed contactors. However, the
experimental values of Richardson-Zaki coefficient
determined here were close to the value of 4.8, commonly
used in the laminar flow regime. The expansion coefficient
and terminal velocity of the adsorbent (Streamline™
DEAE) were theoretically calculated based on correlation
stated in the literature and also experimentally determined.
The adsorbent was also used in batch binding experiments, as
well as in commercial and custom assembled expanded bed
contactors with various feedstocks comprising bovine serum
albumin and egg albumin. This evaluation exhibited good
capacities and adsorption/desorption performance of NBG
column in compare with commercial expanded bed column. The
hydrodynamic behaviour of expanded bed adsorption and the
generic application of simple NBG column and its potential
for the purification and recovery of protein products are
thoroughly discussed.
Key
words:
Expanded bed adsorption, hydrodynamic performance, protein
adsorption, batch binding. |
