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Evaluation of
some bioagents and botanicals in in vitro control of
Colletotrichum destructivum
Akinbode, O.
A* and T. Ikotun
Department of Crop Protection and Environmental Biology,
University of Ibadan, Nigeria.
*Corresponding author. E-mail:
foyedeji@yahoo.com.
Accepted 4 January, 2008 |
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Collectotrichum destructivum
has not been effectively controlled. This led to trials on
the use of bio-agents and botanicals to control the
pathogen. The bio-agents such as Pseudomonas fluorescens,
Bacillus subtilis, Trichoderma harzianum and
Trichoderma pseudokoningii were inoculated as dixenic
culture with the pathogen to monitor antagonistic effect. In
another experiment, botanicals of tobacco (Nicotiana
tabacum) and castor plant (Ricinus communis) were
incorporated as poison in a growth media. Of all the four
bio-agents used, only P. fluorescens was able to
inhibit the growth of the pathogen mycelia significantly by
52%. Though B. subtilis also inhibited the growth of
the pathogen, the effect on the pathogen was not significant
when compared with that of P. fluorescens. The
extracts of R. communis at higher concentration acted
as growth “promoter” to the pathogen; its mycelia had a
better sporulation and fluffiness than that of the
controlled plate. The extract of N. tabacum
significantly controlled the growth of the pathogen at the
highest concentration when compared with other extracts and
the control. In comparison, bio-agents were found to have
better control of the pathogen than the botanicals. P.
fluorescens was the best bio-agent in the control of
C. destructivum.
Key words:
Anthracnose, bioagents, botanicals, inhibitory effect. |
