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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 7 No. 9

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  Mariano AP
  De Angelis DDF

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Scientific Research and Essays
 

African Journal of Biotechnology Vol. 7 (9), pp. 1323–1328, 2 May 2008

ISSN 1684-5315  © 2008 Academic Journals  

 

 

Full Length Research Paper

 

Biodegradability of diesel and biodiesel blends

 

Adriano Pinto Mariano*, Richard Clayton Tomasella, Luciano Marcondes de Oliveira, Jonas Contiero and Dejanira de Franceschi de Angelis

 

Departamento de Bioquímica e Microbiologia - Instituto de Biociências (IB) - Universidade Estadual Paulista (UNESP).

 

*Corresponding author. E-mail: adrianomariano@yahoo.com.br. Fax: +55-1935264176.

 

Accepted 16 February, 2008

 
   Abstract
 

The biodegradability of pure diesel and biodiesel and blends with different proportions of biodiesel (2% (commercial); 5% and 20%) was evaluated employing the respirometric method and the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test. In the former, experiments simulating the contamination of natural environments (soil from a petrol station or water from a river) were carried out in Bartha biometer flasks (250 ml), and used to measure the microbial CO2 production. With the DCPIP test, the capability of three inocula to biodegrade the blends was tested. Results show that although biodiesel is more easily and faster biodegraded than diesel oil, among the blends evaluated (2%, 5% and 20%), only the blend with higher concentration of biodiesel presented biodegradability significantly different from diesel and it was not verified an improvement on the biodegradation of the diesel by means of co-metabolism.

 

Key words: Biodiesel, diesel, blend, biodegradability, bioremediation.

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