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Advance of molecular
marker application in the tobacco research
X. Z. Liu and H. Y.
Zhang*
Biotechnology laboratory; Southwest Forestry College, White
Dragon Temple, Kunming, Yunnan Province, 650224, People’s
Republic of China.
*Corresponding author. E-mail:
zhanghanyao@hotmail.com. Tel: +86-0871-3863022. Fax:
+86-0871-3862178.
Accepted
21 November, 2008 |
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Tobacco
(Nicotiana spp.) is one of the most important
commercial crops in the world.
During the
last two decades, molecular markers have entered the scene
of genetic improvement in different fields of agricultural
research. The principles and characteristics of several
molecular markers such as RFLP, RAPD, AFLP, microsatellites
and minisatellites applied in tobacco genetics and breeding
were reviewed. The application and development of molecular
marker in tobacco genetic research was presented
emphatically in the following areas: evolutionary
genetics, population genetics and genotyping of cultivars,
mapping and tagging of genes, and diversity analysis of
germplasm.
Finally, the perspective of molecular marker’s application
in tobacco genetic breeding in the future was discussed.
Key
words:
Molecular marker, tobacco, genetic and breeding. |