Full Length Research Paper

Plant regeneration through organogenesis and shoot proliferation in Trichodesma indicum (Linn) R. Br. – a medicinal herb

Neelambra Verma, V. Koche, K. L. Tiwari* and S. K. Mishra

School of Life-Sciences, Pt. Ravishankar Shukla University, Raipur 492 010, India.

*Corresponding author. E-mail: neelambara_verma@yahoo.com. Tel: 91-9981513755.

Abbreviations: 2,4-D, 2,4-Dichlorophenoxyacetic acid; BA, N⁶-benzyl aminopurine; IBA, indole-3-butyric acid; MS, Murashige and Skoog medium; NAA, α-naphthalene acetic acid; SH, Schenk and Hildebrandt medium; WPM, woody plant medium.

Accepted 1 September, 2008

Adhahpuspi (Trichodesma indicum, Family – Boraginaceae) is a cross-pollinated species. Its complete regeneration was accomplished through in vitro techniques. The zygotic embryos placed on MS (Murashige and Skoog) medium fortified either with kinetin, BA (N⁶-benzyl Aminopurine) or NAA (α-naphthalene acetic acid) produced callus and adventitious shoots; whereas those placed on MS medium supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) formed callus. On subculture, the nodal pieces produced axillary shoots that were suitable for further propagule proliferation. Rhizogenesis occurred in 60% micro shoots treated with IBA (indole-3-butyric acid) pulse. The regenerated plants successfully acclimatized and started flowering in green house maintained at 30 ± 2^ºC temperature and 70% RH.

Key words: Acclimatization, callus, caulogenesis, micropropagation, pulse treatment.