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Microorganisms’ mediated
reduction of
β-ketoesters
O. Popa1*, Narcisa Babeanu1,
A. Vamanu1, E. Vamanu1 and Diana
Barbulescu2
1Faculty
of Biotechnology, University of Agronomical Sciences and
Veterinary Medicine, 59 Marasti Bld., Bucharest - 011464
Romania.
2National
Institute for Chemical-Pharmaceutical R&D Bucharest, 112
Vitan Road, Bucharest, Romania.
*Corresponding author. E-mail:
ovid_popa@yahoo.com.
Tel: 0040 723693654.
Accepted
22 August, 2008 |
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Several microorganism strains of genera
Saccharomyces, Candida, Hansenula,
Aspergillus and Lactobacillus were screened for
their ability to perform the reduction of
g-chloro-β-ketobutyric acid ethyl ester to
g-chloro-β-hydroxybutyric acid ethyl ester. The optimal
conditions for both stages of the bioprocess were
established. Both stereoisomers of
g-chloro-β-hydroxybutyric acid ethyl ester were
obtained in different biotransformation conditions. While in
aqueous medium the S isomer was obtained, in organic media
the product had predominantly the R configuration.
Key words:
microbial reduction, β-keto-esters, Hansenula polymorpha,
ethyl alcohol. |
