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Biological activities of
Typha capensis (Typhaceae) from Limpopo Province
(South Africa)
Masoko, P.*, Mokgotho, M. P., Mbazima, V. G. and Mampuru,
L. J.
University
of Limpopo, Department of Biochemistry, Microbiology and
Biotechnology, Private Bag X1106, Sovenga, 0727, South
Africa.
*Corresponding author. E-mail:
masokop@ul.ac.za. Tel: +27 15
268 2340. Fax: +27 15 268 3234
Accepted 9
September, 2008 |
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Dried
ground leaves and rhizomes of Typha capensis were
extracted with different solvents of varying polarity
(hexane, dichloromethane, acetone, methanol) to determine
the best extractant for subsequent isolation and
characterization of antibacterial compounds. Some extracts
were active against Escherichia coli and
Enterococcus faecalis, with at least one of them
exhibiting minimum inhibitory concentration values of 0.04
mg/ml. Methanol was the best extractant with an average
minimum inhibitory concentration (MIC) value of 0.75 mg/ml
(rhizome) and 0.21 mg/ml (leaves) for the four pathogens.
E. coli and E. faecalis were the most sensitive
with the average MIC values of 0.53 and 0.42 mg/ml,
respectively. Staphylococcus aureus was the most
resistant pathogen. The MIC values for the positive control
(ampicillin) were ranging from 0.08 to 0.16 mg/ml. Average
total activity, a measure of potency, was highest for
methanol (4498 ml/g) leaves and (1838 ml/g) rhizomes extract
followed by acetone (1795 ml/g) leaves and (1075 ml/g)
rhizome extracts. The most active compounds were at the Rf
value of 0.47 against all pathogens. In some extracts the
antibacterial activity was high enough to consider extracts
for isolation and characterisation of antibacterial
compounds.
Key
words:
Typha capensis, typhaceae, antibacterial,
bioautography. |
