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Isolation,
characterization and identification of actinomycetes from
agriculture soils at Semongok, Sarawak
Jeffrey, L. S. H.
Strategic
Resource Research Centre, Mardi Headquarters, P. O. Box
12301, Pejabat Besar Pos 50774 Kuala Lumpur, Malaysia.
E-mail: shlim@mardi.my.
Tel: +603-8943 7430. Fax no: +603-8943 7677.
Accepted
2 September, 2008 |
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A total of 62 isolates of actinomycetes were isolated from 7
soil samples collected from Agriculture Research Center
Semongok, Sarawak. All 62 isolates exhibited a range of
colony colours (dark grey, grey, dark brown, brownish,
whitish and yellowish white). All the isolates were later
purified and subjected to a few enzymatic screening. Results
indicate that, 48, 46 and 41 isolates showed the ability to
secrete cellulase, lipase and protease respectively. All 62
isolates were then subjected to antimicrobial test using
selected phytopathogens as test strains and it was observed
that 3, 25, 35 and 37 of the isolates showed antagonistic
reaction with Fusarium palmivora, Bacillus
subtilis, Pantoae dipersa and Ralstonia
solanacearum respectively. Six of the most promising
isolates were selected and identified using their 16S rRNA
sequence. All six isolates were identified as
Streptomycetes spp. Further study into the utilization
of the actinomycetes for agriculture industry will be done
to fully utilize these potential microbes for sustainable
agriculture.
Key
words:
Actinomycetes, isolation, characterization, soil, Sarawak. |
