|
In vitro
culture techniques as a tool of sugarcane bud germination
study under salt stress
Ch. B. Gandonou1,2,3, C.
Agbangla2, C. Ahanhanzo2, T. Errabii1,
M. Idaomar1, J. Abrini1 and N.
Skali-Senhaji1*
1Laboratoire
de Biologie et Santé, Université Abdelmalek Essaâdi, Faculté
des Sciences de Tétouan, B.P. 2121 Tétouan, Maroc.
2Laboratoire
de Génétique et des Biotechnologies, Faculté des Sciences et
Techniques (FAST/UAC), 01B.P. 526, Cotonou, Bénin.
3Centre
Béninois de la Recherche Scientifique et Technique (CBRST),
Immeuble Soglo, Maro Militaire, 03B.P. 1665 Cotonou, Bénin.
*Corresponding author. E-mail:
senhajin@hotmail.com.
Fax: 00212 39 99 45 00.
Accepted 25 August, 2008 |
|
Germination was the first stage confronted to soil salinity
and it is important to determine salt effects on this stage.
In this study, we reported an in vitro procedure for
studying sugarcane bud germination and shoot growth under
salt stress with different NaCl concentrations (0, 17, 34,
68 and 102 mM) using cultivar NCo310. Germination percentage
of control was about 92% after 8 days. Germination of buds,
plant fresh and dry mass decreased with increasing salinity.
Data indicated that in vitro culture techniques could
be used to evaluate salt stress effects in sugarcane at the
germination stage.
Key words:
in vitro culture, sugarcane, buds germination, salt
stress, shoot growth. |
