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In vitro
propagation of Amsonia orientalis Decne (Apocynaceae)
Gül Cevahir Öz.*, Elif Yüzbaşioğlu, Osman Erol and Erdal
Üzen
Istanbul
University Botanic Garden, 34134, Süleymaniye, Istanbul
Turkey.
*Corresponding author. E-mail:
cevahirg@istanbul.edu.tr. Tel: 0 212 455 57 00/26808.
Fax: 0 212 522 65 62.
Accepted
16 September, 2008 |
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In
vitro
plant
regeneration was achieved from shoot explants of
Amsonia
orientalis
Decne. The
seeds were germinated aseptically in Petri dishes containing
growth-regulator-free MS medium. The shoot explants of
30-day-old grown seedlings were cultured on MS medium
supplemented with 1 mg/l indole-3- butryric acid (IBA) plus
0.5 mg/l kinetin (Kin) for direct regeneration and
subcultured at three week intervals. While induction of
adventitious buds from shoot explants was achieved
approximately 33% within four weeks, a few roots (around
15%) were induced on the same medium within eight weeks. In
addition, shoot explants were transferred to MS medium
containing 2 mg/l benzyl aminopurine (BAP) plus 0.2 mg/l
naphtalene acetic acid (NAA) for indirect regeneration and
after three weeks, proliferated actively growing calli was
occured.
Key
words:
Amsonia orientalis, propagation, callus formation,
IBA, Kin, NAA, BAP. |
