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Cloning
and characterization of
a cDNA encoding
phytoene synthase (PSY)
in
tea
D. Borthakur, Y. Y. Du,
H. Chen, J. L. Lu, C. Lin, J. J. Dong, J. H. Ye, X. Q. Zheng
and Y. R. Liang*
Zhejiang
University Tea Research Institute, 268 Kaixuan Road,
Hangzhou 310029, China.
*Corresponding author. E-mail:
yrliang@zju.edu.cn.
Fax: +86 571 86971704.
Accepted
9 September, 2008 |
|
Carotenoids are an important group of precursors of
volatile flavour compounds in tea
and phytoene synthase (PSY)
is a key
enzyme during the biosynthesis pathway of carotenoids. A
cDNA encoding PSY in tea shoot
[Camellia
sinensis (L.) O. Kuntze]
was cloned and sequenced in the present study.
The
obtained
tea PSY
cDNA
was 1296
bp
with an open reading frame of 987 bp.
The
predicted protein displayed a sequence of 329 amino acids.
The phylogenetic tree showed
that
the
tea
PSY
was
clustered closely to the PSY of Adonis palaestina,
with an overall amino acid identity of 90%.
Key
words:
Camellia sinensis,
carotenoids,
phytoene
synthase,
gene cloning, sequencing,
phylogenetic tree. |
