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A study on
Cordyceps militaris polysaccharide purification,
composition and activity analysis
Hui Yan*, Dongjie Zhu, Dabao Xu, Jie
Wu and Xuyun Bian
School of Biological and Environmental Engineering, Jiangsu
University of Science and Technology, Zhenjiang, Jiangsu
212018, People’s Republic of China.
*Corresponding author. E-Mail:
yanh1006@163.com.
Accepted 17 October, 2008 |
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In this study, raw Cordyceps militaris polysaccharide
(CPS) was extracted with ultrasonic- assisted extraction
method. Proteins in the raw CPS were removed with the
enzymatic and Sevag methods. After
dialysis, an extraction rate of 0.82% was achieved. Two
components, namely CPS-1 and CPS-2, were obtained from
DEAE-52 ionic exchange chromatography eluted with NaCl
solution at a gradient from 0 to 0.6 mol•L-1. The
ultraviolet scanning demonstrated that CPS-1 did not couple
with protein and nucleic acid, but CPS-2 was coupled with a
few proteins and nucleic acids. After product derivatization
using acid hydrolysis, gas chromatography analysis revealed
that these two CPSs were both composed of mannose and
galactose, with a mole ratio of 0.96:1 and 1.04:1,
respectively. The two CPSs can reverse ethanol-induced
injury in primary cultured rat hepatocytes. Its effect
appeared dose-dependent, having better effect when higher
dose was applied. Its effect is probably due to its
anti-oxidation function.
Key words:
Cordyceps militaris polysaccharide, gas
chromatography, hepatocyte injury, anti-oxidation. |
