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Characterization and
glutenin diversity in tetraploid wheat varieties
in Sulaimanyah by
wheat storage proteins
Nawroz Abdul-razzak Tahir
University of Sulaimanyah, College of Agriculture, Bakrajo,
Sulaimanyah, Iraq. E-mail:
nawroz80@hotmail.com,
nawroz1971@yahoo.fr.
Tel: 009647701965517.
Accepted
3 October, 2008 |
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Important methods applied for the breeding of bread-quality
wheat (Triticum
durum
L.) consist of small-scale bread-quality tests for the
determination of the grain protein content, SDS-sedimentation
volume, thousand weight kernel and kernel diameter. Wheat
grains of six varieties were analyzed. The thousand weight
kernel, protein content, kernel diameter, SDS-sedimentation
volume and SDS index showed a difference significant among
the varieties, whereas the flour yield showed no difference
significant. The quality score and the variability of seed
storage-proteins were analyzed by sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE). The high
molecular weight (HMW) and low molecular weight (LMW)
glutenin subunit band patterns for each variety were
assigned the corresponding Payne numbers and theoretical
quality scores based on those assignments. Three patterns of
HMS-GS are designed: 6+8, 7+8 and 8+20. On the other hand,
two patterns of LMW-GS were detected in the durum varieties.
Genetic diversity of wheat was evaluated by constructing the
dendrogram for high molecular weight (HMW) and low molecular
weight (LMW) gluten subunit bands. It is concluded that seed
storage protein profiles could be useful markers in the
studies of genetic diversity and classification of adapted
cultivars, thereby improving the efficiency of wheat
breeding programs in cultivar development especially in a
developing country.
Key
word:
Durum wheat, glutenin HMW-GS, glutenin LMW-GS, SDS-PAGE,
wheat quality. |
