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Establishment
of a plant regeneration system from callus of Dendrobium
cv. Serdang Beauty
Khosravi, A. R.1, Kadir, M.
A.1, Kazemin, S. B.2, Zaman F.Q3
and De Silva, A. E.3
1Department
of Agrotechnology, Faculty Agriculture, University of Putra,
Malaysia.
2Department
of Crop Science, Faculty Agriculture, University of Putra,
Malaysia.
3Department
of Biology, Faculty Science, University Putra Malaysia.
4School
of Science, Monash University, Sunway Campus, Malaysia.
*Corresponding author. E-mail:
alikho@gmail.com.
Abbreviations:
IAA, Indol-3-acetic acid; IBA, indoe-3-butyric
acid; NAA, α-naphthalenacetic acid; 2,4-D,
2,4-dicolophenoxyactic acid; BAP, N6-benzylaminopurine;
and KIN, 6-fururylaminopurine.
Accepted 30 September, 2008 |
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An in vitro propagation protocol was established for
the Dendrobium Serdang Beauty orchid. The propagation
protocol utilized calli tissues that were successfully
initiated from protocorm-like bodies (PLBs) explants, while
the leaf and root tip explants died. The percentage of
protocorm-like bodies explants responding to calli formation
was 100% in all tested levels of IAA, IBA and NAA auxin
treatments. The highest amount of calli (49.59 gram)
proliferated on MS medium containing 1.5 mg/L IBA. These
calli successfully regenerated on media supplemented with
either KIN or BAP cytokinins and combined treatments of KIN
and IAA (4 mg/L) or NAA (1.5 mg/L). However, media
supplemented with only 1 mg/L KIN was sufficient to produce
significantly high percentage of plantlet formation (80%),
high number of planlets per explant (4-5 plantlets) and high
mean fresh weight per plantlet (11.128 g). These plantlets
were acclimatized on all tested media and obtained
satisfactory rate of plantlet survival (80-100%), mean
number of leaves per plant (4-6 leaves), and mean leaf
length (4 - 5 cm). Among these media, charcoal was
considered the most economical and available material in the
local market. During the development of this protocol,
substantial necrosis of calli were observed when cultures
were treated with 2,4-D and BAP. It was proposed that the
presence of ethylene within the cultures, which is known to
be emitted by plant growth regulators into the micro-climate
of in vitro culture vessels, is the determining
factor of a suitable plant growth regulator for the survival
and growth of the Dendrobium Serdang Beauty calli
cultures in our study.
Keyword:
Auxin, cytokinin, callus, Plbs regeneration, orchid. |
