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Applying the Taguchi
method for optimized fabrication of bovine serum albumin (BSA)
nanoparticles as drug delivery vehicles
Mohsen Jahanshahi*, Ghasem
Najafpour and Mostafa Rahimnejad
Nanobiotechnology Research Lab., School of Chemical
Engineering, Babol University of Technology, Po.Box: 484,
Babol, Iran.
*Corresponding author.
E-mail: mmohse@yahoo.com,
mjahan@nit.ac.ir.
Fax:
+98(0)1113220342.
Accepted
11 January, 2008 |
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The objective of the present study was to optimize the
fabrication of bovine serum albumin (BSA) nanoparticle by
applying the Taguchi method with characterization of the
nanoparticle bioproducts. BSA nanoparticles have been
extensively studied in our previous works as suitable
carrier for drug delivery, since they are biodegradable,
non-toxic and non antigenic. A statistical experimental
design method (Taguchi method with L16 orthogonal array
robust design) was implemented to optimize experimental
conditions of the purpose. Agitation speed, initial BSA
concentration, pH and temperature were considered as process
parameters to be optimized. As the result of Taguchi
analysis in this study, temperature and agitation speed were
the most influencing parameters on the particle size. The
minimum size of nanoparticles (~74 nm) were obtained at 4°C,
pH 7.5, 15 mg ml-1 BSA concentration and
agitation speed of 500 rpm. As for characterization of the
products, Atomic Force microscopy (AFM), Scanning Electron
microscopy (SEM) and Sodium Dodecyl Sulphate- Poly
Acrylamide Gel Electrophoresis (SDS-PAGE) as well as Fourier
Transform Infra-Red (FTIR) techniques were employed.
Key
words:
Bovine serum albumin, nanoparticles, optimization, Taguchi
method, coacervation, atomic Force microscopy. |
