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Integration of C4-specific
ppdk gene of Echinochloa to C3 upland rice and its
photosynthesis characteristics analysis
Jinming Wang
and Runzhi Li*
College of Agronomy,
Shanxi Agricultural University, Taigu 030801, Shanxi, China.
*Corresponding author. E-mail:
rli2001@hotmail.com.
Fax: (86)0354-6288374. Tel: (86)0354-6288374.
Accepted
13 February, 2008 |
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Pyruvate orthophosphate dikinase (PPDK) plays a key role in C4
photosynthetic pathway. The enzymatic reaction is one
of the rate-limiting steps of the C4
photosynthetic pathway. In this paper, the gene encoding
Echinochloa pyruvate orthosphate dikinase (GenBank
accession number: AB289641) was introduced into H65, a
upland rice variety, using an Agrobacterium-mediated
system. Southern hybridization, Northern hybridization and
enzyme activity determination all confirmed the effective
expression of Echinochloa C4 type PPDK in
upland rice with the enzyme activity being elevated 1 - 11
folds. However, no appreciable change demonstrated in carbon
assimilation of the transgenic upland rice though increased
photoinhibition was noted under high light intensity.
Key
words:
Pyrurate orthophosphate dikinase, Echinochloa,
transgenic upland rice, photosynthesis. |
