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Evaluation of the
cytotoxicity interactions between epirubicin and
daunorubicin in HeLa cell cultures
Gül Özcan Arıcan1*,
Uğur Serbes1 and Ercan Arıcan2
1Istanbul University, Science Faculty, Biology Department, Turkey.
2İstanbul University, Science Faculty, Molecular Biology and Genetics
Department, Turkey.
*Corresponding author. E-mail:
gozcan@istanbul.edu.tr. Tel: 00 212 455 57 00 ext.
15093. Fax: 00 212 528 05 27.
Accepted
6 February, 2008 |
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Epirubicin and Daunorubicin, antibiotics which are derivative
of anthracyclines, are used on cancer chemotherapy. In this
study, Epirubicin and Daunorubicin effects on cell kinetics
parameters were examined both single and in combination on
HeLa cell culture that was taken from human servical
carcinoma. The experiments tested by using IC90
doses of Epirubicin and Daunorubicin (0.5 μg/ml and 0.1 μg/ml,
respectively) were applied for 24 and 48 h. Cell kinetics
parameters such as growth rate (WST-1 colorimetric assay),
mitotic and apoptotic index were applied to identify
cytotoxicity that was formed by drugs. In addition, DNA
degradation was examined in agarose gel electrophoresis. The
cytotoxic effect of drugs appeared significant decline of
growth rate and mitotic index, and significant increase of
apoptotic index of HeLa cell line (p<0.05 - 0.001). The
enhancement of the cytotoxicity to Daunorubicin was closely
associated with the DNA degradation.
Key
words:
HeLa cells, cell kinetics, DNA, apoptosis, epirubicin,
daunorubicin. |
