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DNA
polymorphism of butyrophilin gene by PCR-RFLP technique
A. S. Sadr*, M. T. Beigi Nasiri, Kh. Alami-Saeid, J. Fayazi,
H. Roshanfekr and M. Mohammadi
Ramin Agricultural and Natural Resources University,
Mollasani, Ahwaz, Iran.
*Corresponding author. E-mail:
ayehsadr@yahoo.com.
Accepted
18 April, 2008 |
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Butyrophilin plays an important role in secretion of milk
lipid. The present study describes polymorphism of
butyrophilin gene. This is the first study of butyrophilin
gene polymorphism in Najdi cattle of Iran. We used the
polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) technique to screen for DNA
polymorphism in 109 cattle. In all cattle, we amplified an
863 fragment consisting of part of exon 8. The amplified
fragment digested with
HaeIII
restriction endonuclease and subjected to electrophoretic
separation in ethidium bromid-strained 3% agarose gel. Two
alleles were observed,
A and B. Five HaeIII restriction site were found in
the A allele as 371, 231, 185, 83 and 32 bp fragments. In
the B allele, 371 bp were replaced with 338 bp. Three
genotypes were observed, Frequencies were 73.4, 25.68 and
0.92% for AA, AB and BB, respectively. This gives
frequencies of 0.86 and 0.14 for A and B alleles.
Key words:
Butyrophilin gene, polymorphism, PCR-RFLP. |
