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Antimicrobial
activity of the ethanol extract and fractions of the seeds
of Garcinia kola Heckel (Guttiferae)
John, O. Akerele1*,
Osahon, Obasuyi1, Maureen, I. Ebomoyi2
Israel, E. Oboh3,
Osamuyi, H.
Uwumarongie3
1Department
of Pharmaceutical Microbiology, University of Benin, Benin
City, Nigeria.
2Department
of Physiology, University of Benin, Benin City, Nigeria.
3Department
of Pharmacognosy, University of Benin, Benin City, Nigeria.
*Corresponding author: E-mail:
akerelej@yahoo.com
or akerelej@uniben.edu.
Accepted 4 October, 2007 |
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The crude ethanol extract, aqueous and chloroform
fractions of the seeds of Garcinia kola Heckel
(Guttiferae) was investigated for antimicrobial activity.
Agar well diffusion and minimum inhibitory concentration
determinations were the methods employed for the study.
Clinical bacterial and fungal isolates were used as
indicator organisms while standard antimicrobial agents were
included in the study. The crude ethanol extract showed
significant inhibitory activity against clinical isolates of
both Gram positive and Gram negative organisms. It was
active against Staphylococcus aureus, Bacillus
subtilis, Streptococcus viridans, Escherichia
coli, Pseudomonas aeroginosa and Klebsiella
pneumonia. It also showed significant inhibitory
activity against fungi like Penicillium notatum,
Aspergillus niger and Candida albicans.
Both the aqueous and chloroform fractions showed
activity against the clinical isolates of S.
aureus, E. coli and P.
aeruginosa. The MIC values obtained ranged
between 2.5 and 7.5 mg/ml for bacteria and fungi isolates.
The results showed that the crude ethanol extract has broad
spectrum of activity, while the fractions exhibited narrow
spectrum of activity, since they were active against S.
aureus, E. coli, and P.
aeruginosa only. These observations could be the
basis for the usefulness of the seeds of G. kola
in the treatment remedies for microbial infections.
Key words:
Garcinia kola, ethanol extract, antimicrobial
activity. |
