Full Length Research Paper

Effect of Lawsonia inermis treatment on mice with sarcoma

Mehmet Emin Zumrutdal¹, Mehmet Ozaslan²*, Mehmet Tuzcu³, Mehmet Emin Kalender⁵, Kenan Daglıoglu⁴, Atilla Akova¹, Isık Didem Karagöz², İbrahim Halil Kilic², Omer Colak⁵ and Fatih Köksal⁷

¹Departmant of General Surgery, Numune Training Hospital, Adana/Turkey.

²Departmant of Biology, University of Gaziantep, 27310 Gaziantep/Turkey.

³Veterinary Research Institute,  01330 Adana/Turkey.

⁴Animal Research and İmplementation Center,  01330 Adana/Turkey.

⁵Departmant of Oncology, Faculty of Medicine, Gaziantep/Turkey.

⁶Deparment of Biology, University of Cukurova, 01330 Adana/Turkey.

⁷Deparment of Microbiology, University of Cukurova, 01330 Adana/Turkey.

*Corresponding author. E-mail: ozaslanmd@gantep.edu.tr,  ozaslanmd@yahoo.com. Tel:(+90)-342-317-1945. Fax: (+90)-342-360-1200-1032.

Accepted 12 August, 2008

Lawsonia inermis commonly is used as cosmetic material. However many studies had shown that L. inermis had antitumoural, antimicrobial and anti-tuberculostatic effects. The aim of this study was to investigate whether L. inermis can destroy cancer cells by induction of apoptosis due to decreasing of intracellular H⁺ ion level or increasing intracellular free radicals and H₂O₂ levels in cancer cells as a result of oxidative effect or not. We used 70 female Swiss albino mice and divided them into four groups. Group 1 was given only tap water. Group 2 was given only L. inermis. Group 3 was given Ehrlich ascites tumour (EAT) cells + tap water and Group 4 was given EAT + L. inermis. At the result of this study the thickness of subcutaneous lipid tissue, diameters of gluteal mass, the pH levels of gluteal mass, the GSH levels at the liver tissue samples and the MDA levels of the liver tissue samples of these groups were measured. This study showed that, L. inermis can be used as a supplementary agent for cancer treatment.

Key words: L. inermis, Ehrlich ascites tumour, Ehrlich ascites carcinoma, antitumoral.