|
Mouse model in food
allergy: dynamic determination of shrimp allergenicity
Guo Yongchao, Li Zhenxing and Lin Hong*
Food safety
laboratory, Ocean University of China, Qingdao, 266003 PR
China.
*Corresponding author. E-mail:
linhong@ouc.edu.cn,
justso117@163.com.
Tel: 86-532-82032389. Fax: 86-532-82032389.
Accepted
28 August, 2008 |
|
Food allergy is now an important health issue, and there is
urgent need for a developmental approach to identify
allergenic potential of food. We present an approach that
shows some promise for assessment of shrimp allergenicity
using BALB/c strain mice. The mice were immunized by
intraperitoneal injection of shrimp allergen. Mast cell
degranulation in combination with serologic methods was used
to monitor protein allergy. The results showed the method
could continuously reflect the variation of allergic symptom
and actualize dynamic determination of shrimp allergenicity.
Furthermore, it is feasible, sensitive and repeatable. The
approach will provide some valuable reference for
identifying allergenicity of novel food proteins by using
animal model in the future.
Key
words:
Shrimp allergen, allergenicity, mast cell, serology. |
