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Hairy roots induction and
artemisinin analysis in Artemisia dubia and
Artemisia indica
Abdul Mannan1*, Nazia
Shaheen1, Waheed Arshad1, Rizwana
Aleem Qureshi2, Muhammad Zia3 and
Bushra Mirza1
1Department
of Biochemistry, Quaid-i-Azam University, Islamabad,
Pakistan. 2Department of Plant Sciences,
Quaid-i-Azam University, Islamabad, Pakistan. 3Department
of Biotechnology, Quaid-i-Azam University, Islamabad,
Pakistan.
*Corresponding author. E-mail:
abdulmannan_ka@yahoo.com. Tel: 0092-51-90643108.
Accepted
7 July, 2008 |
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Transformation of two Artemisia species (Artemisia
dubia and Artemisia indica) was carried out by
using two Agrobacterium rhizogenes strains LBA 9402
and 8196 for hairy roots production. Induction of hairy
roots was higher in both Artemisia species when
infected with LBA9402 as compared to 8196. When biomass of
roots along with their artemisinin content was evaluated, a
significant effect of Agrobacterium strain on fresh
weight of roots was observed. While artemisinin content was
significantly affected by both plant species as well as
Agrobacterium strains used for infection. However roots
of A. dubia infected either with LBA9402 or 8196
produced maximum artemisinin (0.603% and 0.753,
respectively). When transformed roots were cultured in
liquid medium, highest root fresh weight as well as
artemisinin content (3.9 g and 0.042%, respectively) was
observed in hairy roots of A. indica induced by
Agrobacterium 8196.
Key
words:
Agrobacterium rhizogenes, hairy roots,
transformation, artemisia. |
