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The role of phytohormone
on the production of berberine in the calli cultures of an
endangered medicinal plant, turmeric (Coscinium
fenestratum l.)
Talat Khan1*, David Krupadanam2 and
Syed Yousuf Anwar1
1Department
of Genetics, Osmania University, Hyderabad-500 007, India.
2Department
of Chemistry, Osmania University, Hyderabad-500 007, India.
*Corresponding author. E-mail:
talatkh@yahoo.co.in.
Accepted
26 August, 2008 |
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The present report for the first time describes the results
of study aimed at evaluation of the role of phytohormones on
the production of berberine from in vitro calli
cultures. Calli cultures derived from leaf and petiole
explants were established in MS medium supplemented with
2,4-dichlorophenoxy acetic acid (2,4-D) plus
6-benzylaminopurine
(BAP) and/or kinetin (KN). Berberine an isoquinoline
alkaloid, was isolated from 6 - 7 week old calli cultures.
Media, phytohormones, and explants
used influenced the biomass and berberine content in calli
cultures. Berberine with the retention time of 8.49 min and
enhanced dry weight (1.788%) from the petiole explant is
reported for the first time in this study. The
presence of berberine was first checked by preparative thin
layer chromatography (TLC) and then confirmed by
High Pressure Liquid
chromatography (HPLC) and mass spectrometry. Chemical
structure was determined through proton NMR and 13C
spectra.
Key
words:
Berberine, calli cultures, Coscinium fenestratum,
isoquinolene alkaloid, phytohormones. |
