Full Length Research Paper

Efficient method for the extraction of genomic DNA from wormwood (Artemisia capillaris)

Sayed M. Zain Hasan, Mohammad Shafie B. Shafie* and Ramisah M. Shah

Faculty of Agro-technology and Food Science, Universiti Malaysia Terengganu, 21030 Mengabang Telipot, Kuala Terengganu, Malaysia.

*Corresponding author. E-mail: msshafie@yahoo.com.my.

Accepted 29 May, 2008

Suitable method for isolation of genomic DNA is really important. The best method can make the best result for genetic study. About five differences methods were used amongst which were Sarkosyl Method, CTAB method, Kit Method, SDS Method and Phenol-Chloroform Method. Isolated genomic DNA showed high purity and high quantity in Sarkosyl method compare the other methods. The Sarkosyl DNA isolation method is suitable for restriction digestion and genetic study for Artemisia capillaris.

Key words: Artemisia capillaris, genomic DNA isolation, CTAB, SDS.