|
AFLP
analysis among Ethiopian arabica coffee genotypes
Yigzaw Dessalegn1*, L.
Herselman2 and M. T. Labuschagne2
1IPMS
Project, Bure PLW, P.O. Box 03, Bure, Ethiopia.
2University
of the Free State, Department of Plant Sciences, P.O. Box
339, Bloemfontein 9300, Republic of South Africa.
*Corresponding author. E-mail:
yigzawdessalegn@yahoo.com. Tel: 251-09-18772349,
251-058-7740726.
Accepted 26 June, 2008 |
|
Knowledge of genetic diversity is vital for genetic
improvement and germplasm conservation. The genetic
relationship of 28 Coffea arabica genotypes from
Ethiopia was assessed using 10 Amplified Fragment Length
Polymorphism (AFLP) primer combinations. The level of
polymorphism was 30.9% and 18 markers were unique to 10
genotypes. Pair-wise genetic similarity coefficients ranged
from 0.851 to 0.982, AD1491 being the most divergent and
AD5091 the most closely related to all genotypes. All
genotypes were independently distinguished and did not
cluster according to collection region, demonstrating the
presence of coffee genetic resource diversity within each
region. Results of this study will be utilised for germplasm
collection, conservation as well as in variety development
efforts.
Key words:
Coffea arabica L., DNA-based markers, fingerprinting,
genetic diversity. |
