|
Amelioration of mangosteen
micro propagation through leaf and seed segments
(Garcinia mangostana l.)
Mohammad
Hossein Torabi Sirchi, M. A. Kadir, M. A. Aziz, A. A.
Rashid, Arash Rafat and M. B. Javadi
Department of Agriculture Technology, Faculty of
Agriculture, Universiti Putra Malaysia, 43400 Serdang,
Selangor Darul Ehsan, Malaysia. C/p: +60176066149.
*Corresponding author. E-mail:
hossein955@yahoo.com,
mbjavadi2002@yahoo.com. Tel: 0389464148. Fax:
0389464115.
Accepted 7
May, 2008 |
|
A micro-propagation system was developed for mangosteen (Garcinia
Mangostana L.) using leaf sections and mature seeds.
This study investigated the effect of various modified
Murashige and Skoog (MS) medium supplemented with
6-benzylaminopurin (BAP) alone or in combination with α-naphthalene
acetic acid (NAA) on multiple shoot induction from
leaf and half seed explants. Among
the treatments, leaf explants on medium supplemented with
2.0 mgL־¹ (w/v) of BAP gave the highest percentage of
shoot formation (70.07%), highest number of shoots formed
per explant (46.5%) and showed high mean shoot height (0.48
cm). It was observed that medium with 0.5 mgL־¹
NAA, did not induce shoot organogenesis, but induced callus
in 90% of the leaf explants. Seed explants
gave the
highest percentage of shoot formation (60.2%) and
mean number of shoots per explant (9.5) on medium
supplemented with 20
mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA. However, highest
mean shoot height (0.69 cm) was observed on medium
supplemented with 10 mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA.
Callus induction from seeds was also observed in most of the
treatments. Medium-supplemented 20 mgL־¹ (w/v) NAA gave the
highest percentage of callus formation (83.36%).
Subsequently, roots were induced from the shoots by pretreating
these shoots in various indol-3-butyric acid (IBA) and NAA
concentrations in various MS salt strengths. Root formation
was obtained on one-quarter strength MS salt medium
supplemented with 0.1 mg/L NAA (90.4%). Plantlets were
acclimatized on media composed of soil + sand+ organic
matter+ vermiculite (2: 2: 1: 1) with 80% survival.
Key
words:
Garcinia Mangostana L., 6-benzylaminopurine,
α-naphthalene acetic acid, Indole-3-butyric acid. |
