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Volumetric and viscometric
behaviour of soya bean and gram proteins in aqueous
methotrexate (anticancer drug) solution at 298.15 to 308.15K
Man Singh, Rajesh K. Yadav*
and H. S. Verma
Chemistry
Research Lab, Deshbandhu College, University of Delhi, New
Delhi-110019, and Department of Chemistry J.V. College
Baraut, CCS. University Meerut India.
*Corresponding author. E-mail:
rajeshkr_yadav2003@yahoo.co.in. Tel:
91-011-26217579.
Accepted
28 March, 2006 |
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Apparent molar volumes (Vf)
and viscosities (h)
for 0.00005 to 0.0004 kg.mol-1 (50 to 400
mmol
kg-1) aqueous methotrexate (MTX), gram (Gp) and
soya bean (SBp) proteins, and similar compositions of Gp and
SBp each in 50 to 400
mmol
kg-1 aqueous MTX were determined at 298.15,
303.15 and 308.15K. The Vf
values are positive except aqueous MTX and listed as SBp
> Gp
>
MTX in aqueous and SBp > Gp in MTX solutions, respectively.
It infers weaker hydrophobic heteromolecular interaction of
SBp in binary and ternary systems. The higher
h values of SBp and Gp in MTX than those of aqueous MTX prove
strengthening of hydrophobic interactions of proteins by MTX.
It illustrates the conformational changes of proteins;
slightly higher Vf
values of SBp than of Gp. The MTX confirm higher structural
activity in biological process. The Vf0
of MTX increases with K and decrease with composition. But
with compositions the values continuously decrease with
lower magnitude.
Key
words:
Density, Apparent molal volume, viscosity B coefficient,
methotrexate, soya bean, gram protein. |
