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In vitro
regeneration of Turkish dwarf chickling (Lathyrus cicera
L) using immature zygotic embryo explant
Nurdan Sahin-Demirbag,
Hayrettin Kendir, Khalid Mahmood Khawar*, and
Cemalettin Yasar Ciftci
Department
of Field Crops, Faculty of Agriculture, University of
Ankara, 06110, Diskapi, Ankara, Turkey.
*Corresponding author. E-mail: kmkhawar@gmail.com. Tel: 0090
312 5961540. Fax: 0090 312 3182666).
Abbreviations:
BAP, 6-benzylaminopurine; TDZ, thidiazuron, NAA, α-naphthlene
acetic acid.
Accepted 14
May, 2008 |
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Dwarf chickling (Lathyrus
cicera L) is an important forage legume crop. However,
due to the presence of neurotoxin amino acid (b-N-oxalyl-L-a,b-diaminopropionic
acid) in the dwarf chickling, its cultivation is limited.
There is need to carry out studies for the development of
varieties with reduced or none of this neurotoxin for
beneficial and prolong consumption by animals. The study
reports tissue culture approaches for multiplication of the
plant using thidiazuron (TDZ), 6-benzylaminopurine (BAP),
and α-naphthalene acetic acid (NAA) from immature zygotic
embryos of the plant. The highest number (16.25) of shoots
per explant was recorded on MS medium supplemented with 0.45
mg/l TDZ and 0.4 mg/l ascorbic acid. Much reduced shoot
regeneration was recorded on MS medium containing different
combinations BAP+NAA+ascorbic acid. The TDZ-regenerated
shoots were rooted by pulse treatment with 50 mg/l IBA for 7
min. The plantlets were successfully acclimatized under the
greenhouse conditions.
Key
words:
Immature zygotic embryos,
dwarf chickling, Lathyrus cicera L., axillary shoot
regeneration, rooting. |
