and screening of potential xylanolytic enzyme from litter
Bhathini Vaikuntavasan Pradeep1 Ramaswamy Sathya1
and Jeyaraman Angayarkanni2
of Microbiology, Karpagam Arts and Science College,
Coimbatore -641 021, Tamilnadu, India.
of Biotechnology, Bharathiar University, Coimbatore – 641
046, Tamilnadu, India.
*Corresponding author. E-mail:
28 July, 2006
Consortia of litter degrading fungal species were developed
from different baiting substrates collected in and around
Western ghat forest ecosystem, Coimbatore, Tamilnadu, India.
Fifty-three litter degrading fungal species were isolated by
nylon litterbag technique. The production of endo-b-1,4-xylanase
(1,4-b-D-xylan xylanohydrolase, E.C. 220.127.116.11),
(1,4-b-xylan xylanohydrolase, E.C. 18.104.22.168) and protease was
studied using oat spelt xylan as carbon source. Results
showed that all fifty-three fungal species isolated from
various litter samples produced fairly good xylanolytic
enzyme activity. The xylanase and
activity ranges from 4.41 to 132.20 U and 48.72 to 1510.32
U, respectively. Growth was determined in terms of mycelial
dry weight, which ranged between 0.209 and 1.047 mg/ml. The
protease enzyme activity was from 19.7 to 60.8 U. This is
the first report concerning xylanolytic enzyme production by
the litter degrading fungi, isolated from litter samples.
Litter degrading fungi, xylanolytic enzyme, xylanase,