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Biodegradation of
2,4-dichlorophenol using Mycoplana dimorpha extracts
and evaluation of kinetic parameters
R. Manikandan*,
H. J. Prabhu and P. Sivashanmugam
Department
of Chemical Engineering, National Institute of
Technology,Tiruchirappalli-620015, India.
*Corresponding author. E-mail:
rmanikandan1968@gmail.com,
rmanikandan1968@yahoo.com.
Accepted
24 April, 2008 |
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Twenty seven combinations of process variables were developed
and used to produce crude extracts of Mycoplana dimorpha.
Crude extracts containing 2,4-dichlorophenol degrading
enzymes, were immobilized on sodium alginate beads and
degradation studies was conducted in a packed bed column.
The rate of degradation of 2,4-dichlorophenol by immobilized
crude extracts of was measured at different time intervals
and it was found that 82 to 86% of 2,4-dichlorophenol can be
decomposed with different initial concentrations in 30 min.
The Km and Vmax values were determined.
Key
words:
2,4-Dichlorophenol, degradation, crude extract, Mycoplana
dimorpha, immobilization, packed bed column. |
