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Free radical scavenging
activity and lipoxygenase inhibition of
Woodfordia fructicosa
Kurz and Betula utilis
Wall.
M. V.
Kumaraswamy and S. Satish*
Department
of Studies in Microbiology, University of Mysore,
Manasagangotri, Mysore 570 006, India.
*Corresponding author. E-mail:
umaraswamymv@gmail.com. Tel: +
91821 2419734.
Accepted
16 May, 2008 |
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Woodfordia fructicosa
and Betula utilis were extracted with solvents of
different polarities. Antioxidant activities and
anti-inflammatory activity of the extracts were evaluated by
a 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH),
2,2’-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
and lipoxygenase inhibition assay. Ascorbic acid, gallic
acid and indomethacin were used as reference standards for
DPPH, ABTS and lipoxygenase assays. In both DPPH and ABTS
scavenging activities, methanol and water extract of W.
fructicosa had showed significant scavenging activity
(4.96, 5.08 μg/ml IC50 for DPPH, and 6.4, 7.15 μg
/ml IC50 for ABTS assay). In addition to
antioxidant activity methanol and water extract of W.
fructicosa showed lipoxygenase inhibition activity
(45.22 and 74.24% inhibition at 1.0 mg/ml). The methanol and
water extract of B. utilis was showed DPPH and ABTS
scavenging activity (8.4, 35.08 μg/ml IC50 for
DPPH, and 83.18, 37.14 μg/ml IC50 for ABTS assay)
but very mild activity against lipoxygenase inhibition
activity (18.74 and 28.78% inhibition at 1.0 mg/ml).
The results obtained in the
present study indicate that W. fructicosa can be a
potential source of anti-inflammatory and antioxidant
agents.
Key
words: Woodfordia fructicosa Kurz., Betula utilis Wall.,
lipoxygenase, DPPH, ABTS. |
