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Rapid micro-propagation of
Aloe vera L. via shoot multiplication
Davood Hashemabadi1
and Behzad Kaviani2*
1Department
of Horticulture, Faculty of Agriculture, Islamic Azad
University, Rasht Branch, Rasht, Iran.
2Department
of Biology, Faculty of Agriculture, Islamic Azad University,
Rasht Branch, Rasht, Iran.
*Corresponding author. E-mail:
b.kaviani@yahoo.com.
Tel: 09111777482, + 981314240149. Fax: +981313462255.
Abbreviations:
BA, benzyladenine; NAA, α-naphthaleneacetic acid; MS,
Murashige and Skoog; IBA, 3-indole butyric acid, 2,4-D:
2,4-dichlorophenoxyacetic acid; Kn, kinetin; IAA,
3-indoleacetic acid; ZR, zeatin riboside; 2iPR, 6-(γ, γ-dimethylallyl-amino)
purine riboside; CPPU, N-(2-chloro-4-pyridyl)-N΄-phenylurea.
Accepted
14 May, 2008 |
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Aloe vera L. is a medicinal plant. Regeneration of A. vera in
nature (in vivo) is too slow and insufficient to meet
the industry demand. Therefore, it is necessary to use in
vitro propagation for rapid plant production. Explant
used for the in vitro culture was shoot tip. The
shoot tip explants was disinfected with 2% NaOCl and washed
thoroughly with sterile water. Then, explants were placed on
solid MS medium with the addition of various concentrations
of benzyladenine and α-naphthaleneacetic acid. After 8
weeks, the best proliferation of shoot per explant (9.67)
and the best rooting was shown on the medium supplemented
with 0.5 mg/l benzyladenine + 0.5 mg/l α-naphthaleneacetic
acid. The rooted plantlets were gradually acclimatized in
plastic pots containing a mixture of cocopeat and perlite
(1:1) covered with transparent plastic. About 95% of the
transplanted plantlets survived.
Key
words:
Aloe vera L., micro-propagation, in vitro,
plant growth regulator. |
